IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. IPTG functions by binding to the lac repressor and altering its conformation. …
Puromycin is a protein synthesis inhibitor that causes premature chain termination by acting as an analog of the 3’-terminal end of aminoacyl-tRNA. It has been used to study transcriptional …
Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It plays a role in the citric acid cycle, which is the process by which biochemical …
Many enzymes remain active in the presence of X-100; for example, Proteinase K, remains active in 1% (w/w) solutions of X-100. Triton X-100 can be detected in the parts per million range by …
Stable over wide pH and temperature ranges. Used in detection and protein purification methods because it can bind one molecule of biotin with each subunit. The strong avidin-biotin …
It acts as a hormone precursor since it requires two stages of metabolism: first to 25-hydroxycholecalciferol; then to 1α ,25-dihydroxycholecalciferol. One unit(U.S.P. or …